FastQCFastQC Report
Tue 10 Sep 2024
SRR488142.fastq_short_1

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR488142.fastq_short_1
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC36971.4788000000000001TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC12850.514TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG8480.3392TruSeq Adapter, Index 1 (97% over 49bp)
CTTGGCAACGTGTTCAACCAAGTCGACAACTCTGGTAGAGTAACCGTATT4130.1652No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG2820.11280000000000001TruSeq Adapter, Index 1 (100% over 49bp)
GCCTTGGCAACGTGTTCAACCAAGTCGACAACTCTGGTAGAGTAACCGTA2640.10560000000000001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)