FastQCFastQC Report
Mon 9 Sep 2024
SRR488142.fastq_1

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR488142.fastq_1
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67549405
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC12273941.8170315489825557TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC5277640.7813007383262665TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATG2729600.4040894216610791TruSeq Adapter, Index 1 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG1087990.1610658154575899TruSeq Adapter, Index 1 (100% over 49bp)
CTTGGCAACGTGTTCAACCAAGTCGACAACTCTGGTAGAGTAACCGTATT1017930.15069414749101046No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)